Background:

Sorcin is a highly conserved protein, with 95% homology between hamster and human sorcin sequences. Sorcin has four putative Ca-binding domains, two of which exhibit strong homology to calmodulin “EF hand” motifs. Calcium binding directly to sorcin has been demonstrated by in vitro assays. Sorcin is closely related to members of calpain and sarcoplasmic Ca2+-binding protein subfamilies. Sorcin undergoes calcium-dependent translocation from the cytosol to cellular membranes. Sorcin binds to and modulates ryanodine receptors and is widely distributed including heart and brain tissues. At the subcellular level, sorcin localizes to T-tubule junctions of cardiac sarcoplasmic reticulum.

Specificity:

SRI polyclonal antibody detects endogenous levels of SRI protein.

Alternative names:

Sorcin; 22 kDa protein; CP-22; CP22; V19; SRI

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human SRI.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 22 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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