Background:

Severe acute respiratory syndrome (SARS) coronavirus, a recently emergent infectious agent, shares little homology with previously known coronaviruses with respect to its genes and encoding proteins. Following this pattern, SARS nucleocapsid protein (SARS NC) only weakly resembles analogous proteins of the coronavirus family, and thus estimations of its function prove difficult. In fact, the region encoding SARS NC and other matrix and nucleocapsid proteins more closely resembles avian coronaviruses, while phylogenetic analyses indicate a mammalian origin for the replicase protein. SARS NC expression increases the binding of transcription factors to promotor sequences of c-Fos, ATF2, CREB-1, and FosB, all components of the AP-1 signaling pathway. Other signaling pathways, e.g. the NFκB pathway, are not affected, however, implying that pathway activation by SARS NC is selective, and thus possibly an important target in SARS functional studies.Most viral particles show the appearance of spike-like glycoprotein surface projections, giving rise to the virus' name, which is Latin for crown. These transmembrane spike glycoproteins protrude 20 nanometers from the surface.

Specificity:

SARS polyclonal antibody detects endogenous levels of SARS protein.

Alternative names:

Serine--tRNA ligase, cytoplasmic; Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase; SARS; SERS

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human SARS.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 59 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

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