Background:

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S14, also known as RPS14 or EMTB, is a 151 amino acid component of the small ribosomal 40S subunit. Localized to the cytoplasm, Ribosomal Protein S14 is a member of the S11P family of ribosomal proteins and is highly conserved among several species. Defects in the gene encoding Ribosomal Protein S14 may cause resistance to emetine, a protein synthesis inhibitor found in Chinese hamster ovary cells. Multiple isoforms of this protein exist due to alternative splicing events.

Specificity:

RPS14 polyclonal antibody detects endogenous levels of RPS14 protein.

Alternative names:

40S ribosomal protein S14; RPS14; PRO2640

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RPS14.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 16 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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