Background:

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L14, also known as L14, RPL14, RL14, CTG-B33 or CAG-ISL-7, is a 213 amino acid protein that is a component of the 60S subunit. Localized to the cytoplasm, Ribosomal Protein L14 belongs to the L14e family of ribosomal proteins and functions in protein synthesis. Ribosomal Protein L14 contains a basic region-leucine zipper (bZIP)-like domain and a polymorphic polyalanine tract. The polyalanine tract is believed to participate in transcription regulation. Like most ribosomal proteins, Ribosomal Protein L14 exists as multiple processed pseudogenes that are scattered throughout the genome.

Specificity:

RPL14 polyclonal antibody detects endogenous levels of RPL14 protein.

Alternative names:

60S ribosomal protein L14; CAG-ISL 7; RPL14

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RPL14.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 23 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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