Background:

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L13, also known as RPL13 or BBC1 (breast basic conserved protein 1), is a 211 amino acid protein that is a component of the 60S subunit. Localized to the cytoplasm and expressed ubiquitously, Ribosomal Protein L13 belongs to the L13E family of ribosomal proteins and functions in protein synthesis. In addition, the gene encoding Ribosomal Protein L13 is expressed at high levels in benign breast lesions. Like most ribosomal proteins, Ribosomal Protein L13 exists as multiple processed pseudogenes that are scattered throughout the genome. Due to alternative splicing events and/or alternative polyadenylation, various isoforms exist for Ribosomal Protein L13.

Specificity:

RPL13 polyclonal antibody detects endogenous levels of RPL13 protein.

Alternative names:

60S ribosomal protein L13; Breast basic conserved protein 1; RPL13; BBC1; OK/SW-cl.46

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RPL13.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 24 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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