Background:

Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors. G proteins comprise α, β and γ subunits, and following activation the α subunit binds GTP and dissociates from the βγ complex. A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Gα isoforms by increasing the rate at which they convert GTP to GDP. A subfamily of RGS proteins expressed in the central nervous system contain, in addition to the highly conserved RGS domain, a characteristic GGL domain, or G protein γ subunit-like domain, which mediates binding to Gβ 5 subunits. This subfamily, which includes RGS6, RGS7, RGS9 and RGS11, associates with Gβ 5 to form active GAP complexes that are predominantly localized to the cytosol. RGS/β5 complexes preferentially target Gαo subunit for hydrolysis and inhibit Gβ1γ2-mediated activation of phospholipase C.

Specificity:

RGS7 polyclonal antibody detects endogenous levels of RGS7 protein.

Alternative names:

Regulator of G-protein signaling 7; RGS7

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RGS7.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 58 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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