Background:

Rb (retinoblastoma protein) is a potent transcriptional regulator that is directly involved with events such as entry into cell division and formation of heterochromatin. RBP2 (Retinoblastoma-binding protein 2), also known as RBBP2, JARID1A (Jumonji/ARID domain-containing protein 1A) or KDM5A, is a nuclear protein that belongs to the JARID1 histone demethylase family. Expressed ubiquitously, RBP2 functions as a histone demethylase that, in conjunction with other proteins, binds directly to the viral-binding domain of Rb, thereby regulating Rb-mediated cell proliferation events. In addition, RBP2 can bind to the Rb-interacting protein rhombotin-2 (LMO2) and, through this interaction, can indirectly modulate Rb activity. Via its demethylase activity, RBP2 can remove methyl residues from Histone H3, thus playing a crucial role in the histone code. RBP2 contains one ARID domain, three PHD-type zinc fingers, one JMJN domain and one JMJC domain through which it conveys its enzymatic activity. Multiple isoforms of RBP2 exist due to alternative splicing events.

Specificity:

RBP2 polyclonal antibody detects endogenous levels of RBP2 protein.

Alternative names:

Retinol-binding protein 2; Cellular retinol-binding protein II; CRBP-II; RBP2; CRBP2

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RBP2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 16 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com