Background:

Natural killer (NK) cells attack tumor and infected cells, but the receptors and ligands that stimulate them are poorly understood. Two murine ligands for the lectin-like receptor NKG2D, H60 and retinoic acid early inducible (Rae- 1α, β, γ, δ and ε), are distant relatives of major histocompatibility complex class I molecules. These molecules are encoded by Rae-1 and H60 minor histocompatibility antigen genes on mouse chromosome 10 and show weak homology with MHC class I. Expression of the NKG2D ligands is low or absent on normal, adult tissues; however, they are constitutively expressed on some tumors and upregulated by retinoic acid. Ectopic expression of Rae-1 and H60 confers target susceptibility to NK cell attack. NKG2D binds to H60 with approximately 25-fold higher affinity than to Rae-1. Rae-1 and H60 compete directly for occupancy of NKG2D; therefore, NKG2D can be occupied by only one ligand at a time. Additionally, Rae-1 and H60 ligands of the NKG2D receptor stimulate tumor immunity.

Specificity:

RAE1 polyclonal antibody detects endogenous levels of RAE1 protein.

Alternative names:

mRNA export factor; Rae1 protein homolog; mRNA-associated protein mrnp 41; RAE1; MRNP41

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RAE1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 41 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com