Background:

Bifunctional polynucleotide phosphatase/kinase (PNK), also referred to as polynucleotide kinase-3'-phosphatase, is a member of the DNA 3’ phosphatase family. PNK is the protein expressed by the gene PNKP and contains a 3'-phosphatase domain with similarity to L-2-haloacid dehalogenases and a reported ATP binding site. PNK is a nuclear protein that is involved in DNA repair following damage caused by radiation or oxidation. The protein catalyzes the phosphorylation of DNA at the hydroxy termini but can also dephosphorylate its 3’-phosphate termini. The highest levels of expression of PNK occur in testis, pancreas, spleen, kidney and heart.

Specificity:

PNKP polyclonal antibody detects endogenous levels of PNKP protein.

Alternative names:

Bifunctional polynucleotide; phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; PNKP

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human PNKP.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 57 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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