Background:

PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase), also known as PIMT, is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family and is highly expressed in brain. Functioning as a monomer, PCMT localizes to the cytoplasm and participates in the degradation and/or repair of damaged proteins. More specifically, PCMT1 recognizes isomerized Asp or Asn residues in peptides and proteins and catalyzes the conversion of abnormal L-isoaspartyl and D-aspartyl residues to methyl esters that may then spontaneously hydrolyze to re-form normal aspartyl residues. In congruence with this reaction, PCMT1 converts the methyl donor S-adenosylmethionine (AdoMet) to S-andenosylhomocysteine (AdoHcy). In mice lacking PCMT1, damaged proteins accumulate in a variety of tissues and the ratio between AdoMet and AdoHcy is increased in brain tissue. The phenotypic result is progressive epilepsy and death at an early age.

Specificity:

PCMT1 polyclonal antibody detects endogenous levels of PCMT1 protein.

Alternative names:

Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; L-isoaspartyl protein carboxyl; methyltransferase; Protein L-isoaspartyl/D-aspartyl; methyltransferase; Protein-beta-aspartate methyltransferase; PCMT1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human PCMT1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 25 kDa

Applications:

WB,IF

Usage:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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