Background:

Kynureninase, also known as L-kynurenine hydrolase, is a 465 amino acid cytoplasmic enzyme. Kynureninase is involved in two pathways; the degredation of L-kynurenine and the biosynthesis of the cofactor NAD(+). The main function of kynureninase is to catalyze the cleavage of L-kynurenine into anthranilic acid and of L-3-hydroxykynurenine into 3-hydroxyanthranilic acid, exhibiting a preference for the L-3-hydroxy form. Kynureninase forms a homodimer, uses pyridoxal phosphate as a cofactor and is inhibited by o-methoxybenzoylalanine (OMBA). Kynureninase is widely expressed, with highest levels found in lung, placenta and liver. Deficiency in kynureninase leads to hyperkynureninuria, a disorder characterized by the inability to break down tryptophan to nicotinic acid (vitamin B6). Increased levels of kynureninase activity are observed in systemic and cerebral inflammatory conditions.

Specificity:

KYNU polyclonal antibody detects endogenous levels of KYNU protein.

Alternative names:

Kynureninase; L-kynurenine hydrolase ; KYNU

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human KYNU.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 52 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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