Background:

Glycine N-methyltransferase (GNMT) is a 295 amino acid protein that catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). This process indicates that GNMT probably plays a crucial role in the regulation of tissue concentration of AdoMet and in the metabolism of methionine. Originally identified as a methyl donor, AdoMet is now considered a key metabolite that regulates hepatocyte growth, death and differentiation. Biosynthesis of AdoMet occurs in all mammalian cells as the first step in methionine catabolism in a reaction catalyzed by methionine adenosyltransferase (MAT). Decreased hepatic AdoMet biosynthesis is a consequence of all forms of chronic liver injury. In chronic liver AdoMet deficiency, the liver is predisposed to further injury and can develop spontaneous steatohepatitis and hepatocellular carcinoma. However, impaired AdoMet metabolism, which occurs in patients with mutations of GNMT, can also lead to liver injury.

Specificity:

GNMT polyclonal antibody detects endogenous levels of GNMT protein.

Alternative names:

Glycine N-methyltransferase; GNMT

Species reactivity:

Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human GNMT.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 33 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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