Background:

α-hemoglobin stabilizing protein (AHSP), also designated erythroid associated factor (ERAF), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of α-hemoglobin during normal erythroid cell development.It specifically protects free α-hemoglobin from precipitation in live cells and in solution. It forms a heterodimer with free α-hemoglobin, but not with β-hemoglobin or hemoglobin A (α2-β-2). AHSP localizes to the cytoplasm and is expressed in the blood and bone marrow. The AHSP protein is downregulated in transmissible spongiform encephalopathies (TSEs). AHSP may regulate pathological states of α-hemoglobin excess such as β-thalassemia,a group of hereditary disorders involving the decreased production of normal adult hemoglobin (HbA) that are characterized by a deficiency in the synthesis of β-globin chains.

Specificity:

ERAF polyclonal antibody detects endogenous levels of ERAF protein.

Alternative names:

Alpha-hemoglobin-stabilizing protein; Erythroid differentiation-related factor; Erythroid-associated factor; AHSP; EDRF; ERAF

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human ERAF.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 12 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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