Background:

Tripeptidyl peptidase II (TPPII), is a large intracellular serine exopeptidase with a subtilisin active site . TPPII protein is larger than the 26S proteasome and has a rod-shaped, dynamic supramolecular structure . TPPII is highly conserved among species and it sequentially removes tripeptides from the free amino-terminus of short peptides. TPPII has enhanced activity in proteosome inhibitor-adapted cells and degrades polypeptides by exo- as well as predominantly trypsin-like endoproteolytic cleavage. TPPII participates in the apoptotic pathway triggered by Shigella and is also involved in apoptosis induced by ATP and the protein kinase inhibitor staurosporine . Overexpression of TPPII is sufficient to prevent accumulation of polyubiquitinated proteins and partially restores peptide loading of MHC molecules . TPPII is able to compromise proteosome activity and normalize the rate of intracellular protein breakdown, which is required for normal cellular function and viability.

Specificity:

TPP2 polyclonal antibody detects endogenous levels of TPP2 protein.

Alternative names:

Tripeptidyl-peptidase 2; TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II; TPP2

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human TPP2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 138 kDa

Applications:

WB IF

Usage:

WB: 1:500~1:2000 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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