Background:

Protein-bound methionine residues are among the most susceptible to oxidative stress by biological reactive oxygen species (ROS) such as H2O2, hydroxyl radicals and superoxide ions, which result in the formation of methionine sulfoxide, or Met(O). Methionine sulfoxide reductase (MsrA) is an enzyme that catalyzes the thioredoxin-dependent reduction of Met(O) residues in proteins and in methyl sulfoxide compounds. MsrA is a ubiquitously expressed protein which is found in organisms from yeast to man. Mammalian MsrA is most highly expressed in liver, kidney, macrophages, neutrophils, cerebellum and brain neurons. Oxidation of proteins by ROS is associated with oxidative stress and age-related diseases such as Alzheimer’s disease. Recombinant mammalian MsrA retains enzymatic activity, and overexpression of the protein in yeast and human T cells increases their resistance to oxidative stress. Furthermore, MsrA activity decreases in all regions of the Alzheimer’s disease brain. These findings indicate that MsrA plays an important role in protecting cells against oxidative damage and early cell death.

Specificity:

MSRA polyclonal antibody detects endogenous levels of MSRA protein.

Alternative names:

Mitochondrial peptide methionine sulfoxide reductase; Peptide-methionine (S)-S-oxide reductase; Peptide Met(O) reductase; Protein-methionine-S-oxide reductase; PMSR; MSRA

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human MSRA.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 26 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com