Background:

Aminoacylase-1, also designated N-acyl-L-amino-acid amidohydrolase or ACY-1, is a member of the largest metallopeptidase family called M20A. Aminoacylase-1 is a zinc-binding homodimeric enzyme expressed in kidney, brain, placenta and spleen. It is the most abundant of the aminoacylases. Aminoacylase-1 localizes to the cytoplasm and catalyzes the hydrolysis of N-acylated or N-acetylated amino acids. In addition, Aminoacylase-1 is a sphingosine kinase 1 (SphK1)-interacting protein and may also play a role in regulating responses of the cell to oxidative stress. The gene encoding Aminoacylase-1 is evolutionarily conserved in fish, frog, mouse, rat and human. Deficiency in the Aminoacylase-1 protein may result in defects of brain metabolism and function.

Specificity:

ACY1 polyclonal antibody detects endogenous levels of ACY1 protein.

Alternative names:

Aminoacylase-1; ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human ACY1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 46 kDa

Applications:

WB IHC IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com