Background:

Uev1A, also designated Uev1, UBE2V1 for Ubiquitin-conjugating enzyme E2 variant 1 and CROC1, shows sequence similarity to other ubiquitin-conjugating enzymes, but lacks the conserved cysteine residue critical for their catalytic activity . Therefore, Uev1A does not have ubiquitin-conjugating activity in vitro . However, constitutive expression of exogenous Uev1A in colon carcinoma cells inhibits their capacity to differentiate upon confluence. Studies on recombinant Uev1A show that it localizes to the nucleus, excluding the nucleolar regions . Uev1A functions with TRAF6, a RING domain protein, to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 of ubiquitin . The gene encoding Uev1A maps to human chromosome 20q13.2 .

Specificity:

UBE2V1 polyclonal antibody detects endogenous levels of UBE2V1 protein.

Alternative names:

Ubiquitin-conjugating enzyme E2 variant 1; UEV-1; CROC-1; TRAF6-regulated IKK activator 1 beta Uev1A; UBE2V1; CROC1; UBE2V; UEV1; P/OKcl.19

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human UBE2V1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 16 kDa

Applications:

WB

Usage:

WB 1:500 - 1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com