Background:

HIV-1 Rev is the prototype of a class of retroviral regulatory proteins that control the sequence-specific nuclear export and translation of a class of incompletely spliced HIV-1 mRNAs that encode viral structural proteins. In the absence of Rev, these late viral RNAs remain sequestered in the nucleus until they are either spliced or degraded. The protein designated Rev interacting protein (RIP) or Rev/Rex activation domain-binding protein (Rab) contains 562 amino acids. RIP/Rab has been identified as a cellular cofactor that binds not only to the HIV-1 Rev activation domain, but also to equivalent domains of other Rev and Rex proteins. On the basis of these findings it has been speculated that RIP/Rab is required for the Rev response and thus for HIV-1 replication.

Specificity:

AGFG1 polyclonal antibody detects endogenous levels of AGFG1 protein.

Alternative names:

Arf-GAP domain and FG repeat-containing protein 1; HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein; AGFG1; HRB; RAB; RIP

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human AGFG1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 58 kDa

Applications:

WB IF

Usage:

WB: 1:500~1:2000 IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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