Background:

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L4, also known as RPL4 or RPL1, is a 427 amino acid cytoplasmic protein that is part of the large 60S ribosomal subunit complex. A member of the L4E family of ribosomal proteins, Ribosomal Protein L4 is thought to interact with the multifunctional nucleolar protein RNA helicase II (DDX21) and, through this interaction, may mediate the involvement of DDX21 in rRNA processing. Like most other ribosomal proteins, Ribosomal Protein L4 exists as multiple processed pseudogenes that are scattered throughout the genome.

Specificity:

RPL4 polyclonal antibody detects endogenous levels of RPL4 protein.

Alternative names:

60S ribosomal protein L4; 60S ribosomal protein L1; RPL4; RPL1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human RPL4.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 48 kDa

Applications:

WB,IHC

Usage:

WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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