Background:

ALKB protects DNA and RNA against damage from methylating compounds from the environment by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzymes act by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate, and molecular oxygen as the oxidizing agent. Deficiencies in DNA and RNA repair in mammals are associated with cancer, neurological disease and developmental defects. ALKB plays a role in resistance to anti-cancer drugs which attempt to damage tumor DNA. Escherichia coli ALKB protein belongs to the superfamily of 2-oxoglutarate- and iron(II)-dependent oxygenases.

Specificity:

ALKBH1 polyclonal antibody detects endogenous levels of ALKBH1 protein.

Alternative names:

ABH; ABH1; alkB; AlkB, alkylation repair homolog 1 (E. coli); ALKB1_HUMAN; ALKBH; ALKBH1; Alkylated DNA repair protein alkB homolog 1; Alkylation repair homolog 1; Alkylation repair, alkB homolog; Alpha-ketoglutarate-dependent dioxygenase ABH1; DNA lyase ABH1; DNA oxidative demethylase ALKBH1; hABH;

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human ALKBH1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 43 kDa

Applications:

WB IP

Usage:

WB 1:500 - 1:2000 IP 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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