Background:

Oncostatin M (OSM) is a glycoprotein that was originally isolated from the conditioned medium of U937 human histiocytic leukemia cells that had been induced to differentiate into macrophage-like cells by treatment with phorbol 12-myristate 13 acetate. OSM inhibits the growth of a broad range of human tumor cell lines, but does not influence the growth of normal human fibroblasts. High-affinity binding sites for OSM have been detected on normal and tumor cells, and a receptor has been identified by chemical cross-linking studies. Expression of OSM is greatest in activated monocytic and lymphocytic cell lines and in normal adherent macrophages. Amino acid sequence analysis of OSM has revealed homology with leukemia inhibitory factor (LIF), granulocyte colony stimulating factor (G-CSF) and interleukin 6 (IL-6), all of which affect the growth and differentiation of a broad range of cell types, including those of hematopoietic origin.

Specificity:

OSM polyclonal antibody detects endogenous levels of OSM protein.

Alternative names:

ONCM_HUMAN; Oncostatin M; Oncostatin-M; OSM;

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human OSM.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 28 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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