Background:

BBOX1 (butyrobetaine (γ), 2-oxoglutarate dioxygenase 1), also known as BBH, BBOX, G-BBH (γ-butyrobetaine hydroxylase) or γ-BBH, is a member of the γ-BBH/TMLD family of proteins. Localizing to the cytoplasm and predominantly expressed in kidney, BBOX1 plays a role in the biosynthesis of amines, polyamines and carnitines. More specifically, BBOX1 catalyzes the hydroxylation of γ-butyrobetaine to L-carnitine, a reaction that requires iron and ascorbate as cofactors. This is the last of five steps comprising the L-carnitine biosynthesis pathway and it is important for the proper transport of activated fatty acids across the mitochondrial membrane. Coupled to this reaction, BBOX1 simultaneously catalyzes the oxidative decarboxylation of α-ketoglutarate to succinate.

Specificity:

BBOX1 polyclonal antibody detects endogenous levels of BBOX1 protein.

Alternative names:

2-oxoglutarate dioxygenase; BBH; BBOX 1; BBOX; Bbox1; BODG_HUMAN; Butyrobetaine (gamma) 2 oxoglutarate dioxygenase (gamma butyrobetaine hydroxylase) 1; G BBH; Gamma BBH; Gamma butyrobetaine 2 oxoglutarate dioxygenase 1; Gamma butyrobetaine dioxygenase; Gamma butyrobetaine hydroxylase; Gamma-BBH; Gamma-butyrobetaine; Gamma-butyrobetaine dioxygenase; Gamma-butyrobetaine hydroxylase;

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human BBOX1.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 45 kDa

Applications:

WB

Usage:

WB: 1:500~1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com