Background:

ALKBH3 (alkB, alkylation repair homolog 3), also known as ABH3, PCA1 (prostate cancer antigen 1), DEPC1 or DEPC-1, is a 286 amino acid member of the AlkB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. ALKBH3 is one of many homologs of the Escherichia coli protein, AlkB. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus. It associates with iron and 2-oxoglutarate, coupling the oxidation of substrates to the conversion of 2-oxoglutarate into succinate and CO2. Via oxidative demethylation, ALKBH3 repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA. Its activity is stimulated by ascorbate. Two isoforms exist for ALKBH3 due to alternative splicing of the gene.

Specificity:

ALKBH3 polyclonal antibody detects endogenous levels of ALKBH3 protein.

Alternative names:

ABH3; AlkB homolog 3; alkB, alkylation repair homolog 3 (E. coli); ALKB3_HUMAN; ALKBH3; Alkylated DNA repair protein alkB homolog 3; alkylation repair homolog 3; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3; DEPC 1; DEPC-1; DEPC1; EC 1.14.11.; FLJ43614; mABH3; MGC118790; MGC118792; MGC118793; MGC134125; PCA1; Prostate cancer antigen 1; prostate cancer antigen-1; RP23-375N21.4;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human ALKBH3.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 33 kDa

Applications:

WB

Usage:

WB: 1:500~1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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