Background:

PRPF31 (PRP31 pre-mRNA processing factor 31 homolog), also known as RP11, PRP31 or NY-BR-99, is a ubiquitously expressed protein that localizes to the nucleus and is found in Cajal bodies and speckles. PRPF31 is involved in pre-mRNA splicing and functions as a component of the U4/U6.U5 tri-snRNP (small nuclear ribonucleoprotein) complex. More specifically, PRPF31 is believed to mediate the tethering of the tri-snRNP to the spliceosome (a large ribonucleoprotien responsible for catalyzing the splicing of pre-mRNA), thereby assisting in the assembly of the mature spliceosome. Mutations in the gene encoding PRPF31 result in autosomal dominant retinitis pigmentosa type 11 (RP11), which leads to photoreceptor cell degeneration. RP11 patients initially exhibit a loss of their midperipheral visual field as well as night vision blindness. The disease eventually progresses to the loss of far peripheral visual field and finally the loss of central vision. This suggests that PRPF31 is a key player in the pre-mRNA splicing of photoreceptor-specific genes.

Specificity:

PRPF31 polyclonal antibody detects endogenous levels of PRPF31 protein.

Alternative names:

hPrp 31; hPrp31; NY BR 99; Pre mRNA processing factor 31; Pre mRNA processing factor 31 homolog (yeast); Pre mRNA processing factor 31 homolog; Pre-mRNA-processing factor 31; Precursor mRNA-processing factor 31, S. cerevisiae, homolog of; Protein 61K; PRP 31; PRP31; PRP31 pre mRNA processing factor 31 homolog (yeast); PRP31 pre mRNA processing factor 31 homolog; PRP31_HUMAN; PRPF 31; prpf31; RP 11; RP11; Serologically defined breast cancer antigen NY BR 99; Serologically defined breast cancer antigen NY-BR-99; SNRNP61; U4/U6 small nuclear ribonucleoprotein Prp31; U4/U6 snRNP 61 kDa protein;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human PRPF31.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 55 kDa

Applications:

WB IHC

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com