Background:

TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. Functioning as a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs), TFIID mediates promoter responses to various transcriptional activators and repressors. TAF II p20, also known as TAF12, TAF15, TAF2J or TAFII20, is a 161 amino acid subunit of TFIID that localizes to the nucleus and contains one histone-fold domain. Expressed ubiquitously, TAF II p20 interacts with other members of the TFIID complex and, via this interaction, plays a role in mediating transcriptional activation and repression. Two isoforms of TAF II p20 exist due to alternative splicing events.

Specificity:

TAF12 polyclonal antibody detects endogenous levels of TAF12 protein.

Alternative names:

TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa; TAF15; TAF2J; TAFII 20/TAFII 15; TAFII20; TAFII20/TAFII15; Transcription initiation factor TFIID 20/15 kDa subunits; Transcription initiation factor TFIID subunit 12;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human TAF12.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 18 kDa

Applications:

WB

Usage:

WB: 1:500~1:2000

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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