Background:

The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.

Specificity:

CPT1A polyclonal antibody detects endogenous levels of CPT1A protein.

Alternative names:

Carnitine O palmitoyltransferase 1 liver isoform; Carnitine O palmitoyltransferase I; Carnitine O palmitoyltransferase I liver isoform; Carnitine O-palmitoyltransferase 1; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1A (liver); Carnitine palmitoyltransferase 1A; Carnitine palmitoyltransferase I; Carnitine palmitoyltransferase I liver; CPT 1; CPT I; CPT1; CPT1 L; CPT1-L; Cpt1a; CPT1A_HUMAN; CPTI; CPTI-L; L CPT1; liver isoform;

Species reactivity:

Human,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human CPT1A.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 88 kDa

Applications:

WB,IHC

Usage:

WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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