Background:

Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin α is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Tropomyosin β is encoded by the TPM2 gene, which maps to human chromosome 9p13.3 and undergoes alternative splicing to generate three isoforms, including skeletal muscle (isoform 1), non-muscle/fibroblast TM36/epithelial TMe1 (isoform 2) and non-muscle (isoform 3). Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction.

Specificity:

TPM2 polyclonal antibody detects endogenous levels of TPM2 protein.

Alternative names:

Alpha tropomyosin; AMCD1; Arthrogryposis multiplex congenital distal type 1; Beta; Beta tropomyosin; Cytoskeletal tropomyosin TM30; DA1; FLJ41118; Heat stable cytoskeletal protein 30 kDa; hscp30; HTM alpha; hTM5; MGC14582; MGC3261; MGC72094; NEM1; OK/SW cl.5; Sarcomeric tropomyosin kappa; TM 5; TM3; TM30; TM30nm; TMSA; TMSB; TPM 1; TPM 2; TPM 3; TPM1 alpha; TPM1; TPM1 kappa; TPM2; TPM3; TRK; Tropomyosin 1 alpha; Tropomyosin 1 alpha chain; Tropomyosin 1 alpha chain isoform 6; Tropomyosin 1; Tropomyosin 2; Tropomyosin 2 beta; Tropomyosin 3; Tropomyosin alpha 3 chain; Tropomyosin alpha striated muscle isoform; Tropomyosin beta chain; Tropomyosin gamma;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human TPM2.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~33 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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