Background:

MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5’-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).

Specificity:

MTAP polyclonal antibody detects endogenous levels of MTAP protein.

Alternative names:

5' methylthioadenosine phosphorylase; 5''-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL 249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAP_HUMAN; MTAPase; S methyl 5 thioadenosine phosphorylase; S methyl 5' thioadenosine phosphorylase; S-methyl-5''-thioadenosine phosphorylase;

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human MTAP.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~31 kDa

Applications:

WB IHC IF

Usage:

WB: 1:500~1:2000 IHC/IF: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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