Background:

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.

Specificity:

POLR2I polyclonal antibody detects endogenous levels of POLR2I protein.

Alternative names:

DNA directed RNA polymerase II 14.5 kDa polypeptide; DNA directed RNA polymerase II polypeptide I; DNA directed RNA polymerase II subunit I; DNA directed RNA polymerase II subunit RPB9; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; hRPB14.5; hRPB220; hsRPB1; MGC75453; POLR2; POLR2I; POLRA; Polymerase (RNA) II (DNA directed) polypeptide I 14.5kDa; RNA polymerase II 14.5 kDa subunit; RNA polymerase II subunit B9; RPB1; RPB14.5; RPB220; RPB9; RPB9_HUMAN; RPBh1; RpIILS; RPO2; RPOL2;

Species reactivity:

Human,Mouse

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human POLR2I.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~15 kDa

Applications:

WB

Usage:

WB: 1:500~1:2000 IHC: 1:50~1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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