Background:

17b-hydroxysteroid dehydrogenase type 1 (17b-HSD) catalyzes the final step in the formation of estradiol and testosterone from estrone and androstenedione, respectively. Ovarian granulosa cells and breast tissue both express 17b-HSD. Other tissues that express 17b-HSD include testis, placenta, uterus, prostate and adipose tissue. 17b-HSD functions as a homodimer and prefers NADP(H) over NAD(H) for oxidation and reduction. The gene encoding human 17b-HSD maps to chromosome 17q12-q21. The importance of 17b-HSD to estradiol production suggests the specific inhibition of 17b-HSD may aid in breast cancer therapy. Breast cancer patients with an amplification of 17b-HSD expression statistically have a worse outcome than those without. 17b-HSD amplification in tamoxifen-treated patients correlates to decreased breast cancer survival.

Specificity:

HSD17B1 polyclonal antibody detects endogenous levels of HSD17B1 protein.

Alternative names:

EDH17B2;EDHB17;HSD17;SDR28C1

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human HSD17B1

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

35kDa

Applications:

WB IHC IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

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