Background:

Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of cognate tRNA in the initial step of ribosome-dependent protein biosynthesis. DaRS is part of a multisubunit complex of aminoacyl-tRNA synthetases and is involved in the transfer of Asp-tRNA to EF-1 α1 (elongation factor α 1). The N-terminus of DaRS in vertebrates is a newly evolved structure that contains a putative amphiphilic helix and is dissimilar between different species. The N-terminal extension acts as a switch that, when in its stretched form, reduces the rate of dissociation of Asp-tRNA from DaRS, thereby providing enough time for EF-1 α1 to interact with Asp-tRNA. This suggests that the N-terminus of DaRS plays a critical role in its catalytic function. DaRS contains two phosphorylations sites, forms homodimers and localizes to the cytoplasm.

Specificity:

DARS polyclonal antibody detects endogenous levels of DARS protein.

Alternative names:

Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; DARS; PIG40

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant full length Human DARS.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 57 kDa

Applications:

WB,IHC

Usage:

WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

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