Background:

Sm and Sm-like (LSm) proteins form donut-shaped heptameric complexes that are involved in various steps of RNA metabolism. LSm proteins facilitate RNA protein interactions and structural changes that are required during ribosomal subunit assembly. LSm1, also designated U6 snRNA-associated Sm-like protein or small nuclear ribonuclear CaSm, binds specifically to the 3'-terminal U-tract of U6 snRNA. Human LSm1 localizes to the cytoplasm in small, discrete foci. These foci are also the localization site for the mRNA decapping enzyme Dcp1/2 and the exonuclease Xm1. LSm1 is naturally overexpressed in pancreatic cancer as well as in certain breast cancer cell lines. The downregulation of LSm1 is involved in the progression of prostate cancer.

Specificity:

LSM1 polyclonal antibody detects endogenous levels of LSM1 protein.

Alternative names:

U6 snRNA-associated Sm-like protein LSm1; Cancer-associated Sm-like; Small nuclear ribonuclear CaSm; CASM

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human LSM1

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 17 kDa

Applications:

WB IHC

Usage:

WB 1:1000 - 1:3000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com