Background:

Ribonuclease P (RNase P) and Ribonuclease MRP (RNase MRP) are small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. RNase P and RNase MRP, which accumulate in the nucleolus, have a similar RNA component and also have several protein subunits in common. RNase P, which consists of a complex of an RNA species, POP1, POP5, and at least 7 Rpps, removes the 5' leader sequences from precursor tRNA molecules. Rpp25 (ribonuclease P/MRP 25 kDa subunit) is a 199 amino acid nuclear protein that belongs to the histone-like Alba family and functions as a component of nuclear RNase P and RNase MRP ribonucleoproteins.

Specificity:

RPP25 polyclonal antibody detects endogenous levels of RPP25 protein.

Alternative names:

Ribonuclease P protein subunit p25; RNase P protein subunit p25

Species reactivity:

Human

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human RPP25.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 21 kDa

Applications:

WB,IHC

Usage:

WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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