Background:

AMT (Aminomethyltransferase), also known as GCST (Glycine cleavage system T), GCE or NKH, is a 403 amino acid mitochondrial protein that is one of four components of the glycine cleavage system. The glycine cleavage system is comprised of AMT (known as Protein T), GCSH (known as Protein H), DLD (known as Protein L) and GLDC (known as Protein P), all of which work together to catalyze the cleavage and degradation of glycine. Expressed ubiquitously, AMT plays a crucial role in glycine degradation pathway, specifically catalyzing the creation of 5,10-methylenetetrahydrofolate.

Specificity:

AMT polyclonal antibody detects endogenous levels of AMT protein.

Alternative names:

GCST; GCVT; Aminomethyltransferase

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human AMT.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 44 kDa

Applications:

WB,IHC,IF

Usage:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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