Background:

OTC (ornithine carbamoyltransferase), also known as OTCase, is a 354 amino acid protein that belongs to the ATCase/OTCase family of proteins. Expressed in liver and intestinal mucosa, OTC localizes to the mitochondrial matrix and exists as a homotrimer. Specifically, OTC plays a vital role in the urea cycle, catalyzing the second step in this pathway: the formation of L-citrulline from L-orthinine and carbamoyl phosphate. In humans, the urea cycle is an important pathway to detoxification of ammonia. Mutations in the gene encoding OTC are associated with the X-linked disorder OTCD (ornithine carbamoyltransferase deficiency).

Specificity:

OTC polyclonal antibody detects endogenous levels of OTC protein.

Alternative names:

Ornithine transcarbamylase; OTCase

Species reactivity:

Human,Mouse,Rat

Host:

Rabbit

Ig type:

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen:

Recombinant protein of human OTC.

Purification & Purity:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Molecular weight:

~ 40 kDa

Applications:

WB IHC

Usage:

WB 1:1000 - 1:4000 IHC 1:50 - 1:100

Storage:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Precautions:

For research use only, not for use in diagnostic procedure.

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