Host:

E.coli

Tag:

His-tag

AA Sequence:

AGCAGTAGCAATGTGGAAGTGTTCATTCCGGTGAGCCAGGGTAATACCAATGGCTTCCCGGCAACCGCAAGCAATGATCTGAAAGCCTTCACCGAAGGCGCAGTGCTGAGCTTCCATAATATCTGTTATCGCGTTAAACTGAAAAGTGGCTTCCTGCCGTGTCGTAAACCGGTGGAAAAAGAAATTCTGAGTAATATTAACGGCATCATGAAACCGGGCCTGAATGCAATTCTGGGTCCGACCGGCGGCGGTAAAAGTAGCCTGCTGGATGTGCTGGCCGCACGCAAAGATCCGAGTGGTCTGAGCGGTGATGTGCTGATTAATGGTGCCCCGCGCCCGGCAAACTTCAAATGCAATAGCGGTTATGTGGTGCAGGATGATGTGGTTATGGGTACCCTGACCGTGCGTGAAAATCTGCAGTTCAGTGCCGCCCTGCGTCTGGCAACCACCATGACCAATCATGAAAAAAATGAACGTATCAACCGCGTTATTCAGGAACTGGGTCTGGATAAAGTTGCCGATAGCAAAGTTGGCACCCAGTTCATTCGCGGTGTTAGCGGTGGCGAACGCAAACGTACCAGTATTGGCATGGAACTGATTACCGATCCGAGCATTCTGTTCCTGGATGAACCGACCACCGGCCTGGATAGCAGTACCGCAAATGCAGTGCTGCTGCTGCTGAAACGTATGAGCAAACAGGGTCGTACCATTATCTTCAGCATTCATCAGCCGCGTTATAGTATCTTCAAACTGTTCGATAGTCTGACCCTGCTGGCAAGTGGTCGCCTGATGTTCCATGGTCCGGCCCAGGAAGCACTGGGTTACTTCGAAAGCGCAGGCTATCATTGTGAAGCCTATAATAATCCGGCCGACTTCTTCCTGGATATTATTAATGGTGATAGTACCGCAGTGGCCCTGAATCGCGAAGAAGACTTCAAAGCCACCGAAATTATTGAACCGAGTAAACAGGATAAACCGCTGATTGAAAAACTGGCCGAAATCTATGTTAATAGTAGCTTCTATAAGGAGACCAAAGCCGAACTGCATCAGCTGAGCGGTGGTGAAAAAAAAAAAAAGATTACCGTGTTCAAGGAGATTAGTTATACCACCAGCTTCTGCCATCAGCTGCGCTGGGTTAGCAAACGCAGCTTCAAAAATCTGCTGGGCAATCCGCAGGCCAGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~43kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells. ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either full-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney. ABCG2 expression has also been observed in stem cell populations, particularly in hematopoietic and neuronal stem cells and is downregulated with differentiation.

Note:

For research use only, not for use in diagnostic procedure.