Host:

E.coli

Tag:

His-tag

AA Sequence:

GAAAAGCCGGTGTATGAACTGCAGGTTCAGAAAAGCGTGACCGTGCAGGAAGGCCTGTGTGTGCTGGTGCCGTGTAGCTTCAGCTATCCGTGGCGCAGCTGGTATAGTAGTCCGCCGCTGTATGTGTATTGGTTCCGCGATGGTGAAATTCCGTATTATGCCGAAGTTGTTGCCACCAATAATCCGGATCGCCGTGTTAAACCGGAAACACAAGGTCGCTTCCGCCTGCTGGGTGATGTGCAGAAAAAAAATTGTAGCCTGAGCATTGGCGATGCACGTATGGAAGATACCGGTAGCTACTTCTTCCGTGTTGAACGTGGCCGTGATGTTAAATATAGTTATCAGCAGAATAAGCTGAACCTGGAAGTTACCGCACTGATTGAAAAACCGGATATTCACTTCCTGGAACCGCTGGAAAGCGGTCGTCCGACCCGTCTGAGCTGCAGTCTGCCGGGTAGTTGCGAAGCAGGCCCGCCGCTGACCTTCAGTTGGACCGGTAATGCCCTGAGCCCGCTGGATCCGGAAACCACCCGCAGCAGTGAACTGACCCTGACCCCGCGCCCGGAAGATCATGGTACCAATCTGACCTGCCAGATGAAACGTCAGGGTGCCCAGGTGACCACCGAACGTACCGTTCAGCTGAATGTTAGTTATGCCCCGCAGACCATTACCATCTTCCGCAATGGCATTGCCCTGGAAATTCTGCAGAATACCAGCTATCTGCCGGTTCTGGAAGGTCAGGCCCTGCGCCTGCTGTGCGATGCCCCTAGTAATCCGCCGGCACATCTGAGTTGGTTCCAGGGCAGTCCGGCACTGAATGCAACCCCGATTAGCAATACCGGCATTCTGGAACTGCGCCGTGTGCGCAGTGCAGAAGAAGGTGGCTTCACCTGTCGCGCACAGCATCCGCTGGGCTTCCTGCAGATCTTCCTGAATCTGAGTGTGTATAGTCTGCCGCAGCTGCTGGGCCCGAGTTGTAGCTGGGAAGCCGAAGGTCTGCATTGCCGCTGTAGCTTCCGTGCCCGTCCGGCACCGAGCCTGTGTTGGCGTCTGGAAGAAAAACCGCTGGAAGGTAATAGCAGTCAGGGCAGCTTCAAAGTTAATAGCAGTAGTGCAGGCCCGTGGGCAAATAGCAGTCTGATTCTGCATGGCGGTCTGAGCAGCGATCTGAAAGTGAGTTGCAAAGCATGGAATATCTATGGCAGTCAGAGTGGTAGTGTGCTGCTGCTGCAGGGTCGCAGCAATCTGGGTACCGGTGTTGTGCCGGCAGCCCTG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~47kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Two families of mammalian lectin-like adhesion molecules bind glycoconjugate ligands in a sialic acid-dependent manner: the selectins and the sialoadhesins. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members recognizing sialylated ligands. Siglec-5 binds equally to α2,3-linked and α2,6-linked sialic acid. There exist four isoforms of hSiglec-5 possessing three (hSiglec-5-3L and -3C) or four (hSiglec-5-4L and -4S) extracellular domains linked to long (hSiglec-5-3L and -4L) or short (hSiglec-5-4S) cytoplasmic tails or existing as a soluble isoform (hSiglec-5-3C). Siglec-5 is expressed by monocytes and neutrophils, but is absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec-5 may play a role in the diagnosis and monitoring of acute myeloid leukemia (AML).

Note:

For research use only, not for use in diagnostic procedure.