Host:

E.coli

Tag:

His-tag

AA Sequence:

CTGGCTCCTCGTCGCTGTCCGGCCCAGGAAGTTGCCCGTGGTGTGCTGACCAGCCTGCCGGGTGATAGCGTTACCCTGACCTGCCCGGGCGTTGAACCGGAAGATAATGCAACCGTTCATTGGGTGCTGCGTAAACCGGCCGCAGGCAGCCATCCGAGCCGTTGGGCTGGTATGGGTCGCCGTCTGCTGCTGCGCAGCGTGCAGCTGCATGATAGCGGCAATTATAGTTGCTATCGTGCCGGTCGCCCGGCAGGCACCGTTCATCTGCTGGTGGATGTGCCGCCGGAAGAACCGCAGCTGAGCTGCTTCCGCAAAAGCCCGCTGAGTAATGTGGTGTGCGAATGGGGCCCGCGTAGTACCCCGAGTCTGACCACCAAAGCCGTTCTGCTGGTGCGCAAATTCCAGAATAGCCCGGCCGAAGACTTCCAGGAACCGTGCCAGTATAGTCAGGAAAGTCAGAAATTCAGTTGCCAGCTGGCAGTGCCGGAAGGCGATAGCAGCTTCTATATTGTGAGTATGTGTGTTGCCAGCAGCGTTGGTAGCAAATTCAGTAAAACACAAACCTTCCAGGGTTGTGGCATTCTGCAGCCGGATCCGCCGGCCAATATTACCGTTACCGCAGTTGCCCGCAATCCGCGCTGGCTGAGTGTGACCTGGCAGGATCCGCATAGCTGGAATAGCAGCTTCTACCGCCTGCGCTTCGAACTGCGTTATCGCGCAGAACGCAGTAAAACCTTCACCACCTGGATGGTTAAAGATCTGCAGCATCATTGCGTGATTCATGATGCATGGAGTGGCCTGCGTCATGTGGTTCAGCTGCGCGCACAGGAAGAATTCGGCCAGGGTGAATGGAGTGAATGGAGCCCGGAAGCAATGGGCACCCCGTGGACCGAAAGCCGCAGCCCTCCGGCTGAAAATGAAGTGAGCACCCCGATGCAGGCACTGACCACCAATAAAGATGATGATAATATTCTGTTCCGCGATAGCGCCAATGCCACCAGTCTGCCGGTTCAGGATAGTAGCAGCGTGCCGCTGCCG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~44kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

The IL-6 receptor is a heterodimeric complex that consists of a ligand-binding IL-6 receptor α (IL-6Rα) subunit and a signaling component, gp130. Binding of IL-6 to IL-6Rα results in dimerization of receptor with gp130 and subsequent STAT3 activation (1). IL-6Rα is cleaved from the cell surface by ADAM17. In humans, soluble IL-6Rα is also generated via alternatively spliced mRNA. Soluble IL-6Rα binds to IL-6 and can stimulate signaling via membrane bound gp130 in a process known as “trans-signaling”. It is through trans-signaling that IL-6 stimulates cells that do not express membrane bound IL-6Rα.

Note:

For research use only, not for use in diagnostic procedure.