Host:

E.coli

Tag:

His-tag

AA Sequence:

CAGCCTAGTGTTAGCCCGGGTGAACCGAGCCCGCCGAGCATTCATCCGGGCAAAAGCGATCTGATTGTTCGTGTTGGTGATGAAATTCGTCTGCTGTGCACCGATCCGGGCTTCGTGAAATGGACCTTCGAAATTCTGGATGAAACCAATGAAAATAAGCAGAATGAATGGATTACCGAAAAAGCAGAAGCCACCAATACCGGCAAATATACCTGCACCAATAAACATGGTCTGAGTAATAGTATCTATGTGTTCGTTCGCGATCCGGCCAAACTGTTCCTGGTGGATCGTAGCCTGTATGGTAAAGAAGATAATGATACCCTGGTGCGCTGTCCGCTGACCGATCCGGAAGTTACCAATTATAGTCTGAAAGGTTGCCAGGGCAAACCGCTGCCGAAAGATCTGCGCTTCATTCCGGATCCGAAAGCAGGCATTATGATTAAAAGCGTTAAACGCGCATATCATCGTCTGTGTCTGCATTGCAGCGTGGATCAGGAAGGTAAAAGTGTTCTGAGCGAAAAATTCATTCTGAAAGTGCGCCCGGCATTCAAAGCAGTTCCGGTTGTGAGCGTGAGTAAAGCAAGTTATCTGCTGCGCGAAGGCGAAGAATTCACCGTGACCTGTACCATTAAAGATGTGAGTAGCAGCGTGTATAGTACCTGGAAACGTGAAAATAGTCAGACCAAACTGCAGGAAAAATATAATAGTTGGCATCATGGTGACTTCAATTATGAACGTCAGGCCACCCTGACCATTAGCAGCGCCCGCGTTAATGATAGCGGCGTGTTCATGTGTTATGCAAATAATACCTTCGGTAGCGCCAATGTGACCACCACCCTGGAAGTTGTGGATAAAGGCTTCATTAATATCTTCCCGATGATTAATACCACCGTGTTCGTGAATGATGGTGAAAATGTGGATCTGATTGTGGAATATGAAGCATTCCCGAAACCGGAACATCAGCAGTGGATCTATATGAATCGCACCTTCACCGATAAATGGGAAGATTATCCGAAAAGCGAAAATGAAAGCAATATTCGTTACGTGAGTGAACTGCATCTGACCCGCCTGAAAGGCACCGAAGGCGGCACCTATACCTTCCTGGTGAGTAATAGCGATGTGAATGCAGCAATTGCCTTCAATGTGTATGTGAATACCAAACCGGAAATTCTGACCTATGATCGCCTGGTGAATGGCATGCTGCAGTGCGTTGCAGCCGGCTTCCCGGAACCGACCATTGATTGGTACTTCTGCCCGGGTACCGAACAGCGTTGTAGCGCCAGTGTGCTGCCGGTGGATGTTCAGACCCTGAATAGCAGCGGCCCGCCGTTCGGTAAACTGGTTGTTCAGAGCAGTATTGATAGTAGTGCCTTCAAACATAATGGCACCGTGGAATGCAAAGCCTATAATGATGTGGGTAAAACCAGCGCATACTTCAACTTCGCCTTCAAAGGTAATAATAAAGAACAGATTCACCCGCATACCCTGTTCACCCCG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~55kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo.

Note:

For research use only, not for use in diagnostic procedure.