Host:

E.coli

Tag:

His-tag

AA Sequence:

GCAATGTTCATGGTGAAAAATGGCAATGGTACCGCATGCATTATGGCCAACTTCAGTGCCGCATTCAGTGTTAATTATGATACCAAAAGCGGTCCGAAAAATATGACCTTCGATCTGCCGAGCGATGCAACCGTGGTTCTGAATCGCAGCAGTTGTGGCAAAGAAAATACCAGCGATCCGAGCCTGGTGATTGCATTCGGTCGTGGTCATACCCTGACCCTGAACTTCACCCGTAATGCCACCCGTTATAGTGTGCAGCTGATGAGCTTCGTGTATAATCTGAGTGATACCCATCTGTTCCCGAATGCAAGTAGCAAAGAAATTAAAACCGTTGAAAGTATCACCGATATTCGTGCAGATATTGATAAAAAATACCGCTGCGTGAGTGGTACCCAGGTGCACATGAATAATGTGACCGTTACCCTGCATGATGCCACCATTCAGGCATATCTGAGTAATAGTAGCTTCAGCCGCGGCGAAACCAGATGTGAACAGGATCGCCCGAGTCCGACCACCGCACCGCCTGCACCTCCGTCACCGAGTCCGAGCCCGGTTCCGAAAAGCCCGAGTGTGGATAAATATAATGTTAGCGGTACCAATGGTACCTGCCTGCTGGCAAGTATGGGCCTGCAGCTGAATCTGACCTATGAACGTAAAGATAATACCACCGTGACCCGTCTGCTGAATATTAATCCGAATAAAACCAGCGCCAGCGGTAGCTGTGGTGCACATCTGGTGACCCTGGAACTGCATAGTGAAGGTACCACCGTGCTGCTGTTCCAGTTCGGTATGAATGCAAGTAGTAGCCGCTTCTTCCTGCAGGGTATTCAGCTGAATACCATTCTGCCGGATGCACGCGATCCGGCCTTCAAAGCAGCCAATGGCAGCCTGCGCGCACTGCAGGCAACCGTTGGCAATAGCTATAAATGCAATGCCGAAGAACATGTGCGCGTTACCAAAGCATTCAGCGTTAATATCTTCAAAGTGTGGGTTCAGGCATTCAAAGTTGAAGGCGGTCAGTTCGGTAGTGTTGAAGAATGCCTGCTGGATGAAAATAGCATG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~37kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis. The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes. LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking.

Note:

For research use only, not for use in diagnostic procedure.