Host:

E.coli

Tag:

His-tag

AA Sequence:

GAAAGCGGTTATGCCCAGAATGGTGATCTGGAAGATGCAGAACTGGATGATTATAGCTTCAGTTGTTATAGTCAGCTGGAAGTGAATGGCAGCCAGCATAGCCTGACCTGCGCATTCGAAGATCCGGATGTTAATATTACCAATCTGGAATTCGAAATCTGCGGCGCACTGGTTGAAGTGAAATGCCTGAACTTCCGCAAACTGCAGGAAATCTACTTCATTGAAACCAAAAAATTCCTGCTGATTGGTAAAAGCAATATCTGTGTTAAAGTGGGTGAAAAAAGCCTGACCTGTAAAAAAATTGATCTGACCACCATTGTGAAACCGGAAGCACCGTTCGATCTGAGTGTGGTGTATCGTGAAGGCGCAAATGACTTCGTGGTGACCTTCAATACCAGTCATCTGCAGAAAAAATATGTTAAAGTGCTGATGCATGACGTGGCATATCGCCAGGAAAAAGATGAAAATAAATGGACCCATGTTAACCTGAGTAGTACCAAACTGACCCTGCTGCAGCGTAAACTGCAGCCGGCAGCCATGTATGAAATTAAAGTTCGTAGCATTCCGGATCATTACTTCAAAGGCTTCTGGAGCGAATGGAGTCCGAGTTATTACTTCCGCACCCCGGAAATTAATAATAGTAGTGGTGAAATGGAC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~28kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Interleukin 7 (IL-7) was originally described as a factor capable of inducing in vitro proliferation of pre-B cells from marrow cultures. The IL-7 gene encodes a protein 177 amino acids in length. IL-7 exerts its biological function through the IL-7 receptor which is expressed on pre-B cells, thymocytes and bone marrow-derived macrophages. The IL-7 receptor is composed of an IL-7 receptor-specific chain and the IL-2 receptor γ chain common to the IL-2, IL-4, IL-7, IL-9 and IL-15 receptors. IL-7 stimulation leads to the activation of Janus tyrosine kinase family members JAK1 and JAK3. Other studies have shown that in T cells, the IL-7 receptor-specific chain associates with the Src kinases family Lck and Fyn. IL-7 induces phosphorylation of insulin receptor substrate-1 (IRS-1) and Insulin receptor substrate-2 (IRS-2), originally called 4PS.

Note:

For research use only, not for use in diagnostic procedure.