Host:

E.coli

Tag:

His-tag

AA Sequence:

CAGGATAGCCGCGGCTGCGCACGCTGGTGCCCTCAAAATAGTAGTTGCGTTAATGCAACCGCATGCCGTTGTAATCCGGGCTTCAGCAGCTTCAGCGAAATTATTACCACCCCGACCGAAACCTGTGATGATATTAATGAATGCGCAACCCCGAGTAAAGTGAGTTGCGGCAAATTCAGCGATTGCTGGAATACCGAAGGCAGCTATGATTGCGTGTGTAGCCCGGGCTATGAACCGGTGAGTGGCGCAAAAACCTTCAAAAATGAAAGCGAAAATACCTGTCAGGATGTGGATGAATGTCAGCAGAATCCGCGTCTGTGTAAAAGCTATGGCACCTGCGTTAATACCCTGGGTAGCTATACCTGCCAGTGCCTGCCGGGCTTCAAATTCATTCCGGAAGATCCGAAAGTGTGCACCGATGTTAATGAATGCACCAGTGGCCAGAATCCGTGTCATAGTAGTACCCATTGTCTGAATAATGTGGGCAGTTATCAGTGCCGTTGTCGCCCGGGCTGGCAGCCTATTCCGGGTAGTCCGAATGGTCCGAATAATACCGTGTGTGAAGATGTTGATGAATGCAGCAGTGGTCAGCATCAGTGCGATAGTAGCACCGTGTGCTTCAATACCGTGGGCAGTTACAGTTGTCGTTGCCGTCCGGGTTGGAAACCGCGTCATGGTATTCCGAATAATCAGAAAGATACCGTGTGTGAGGATATGACCTTCAGTACCTGGACCCCGCCGCCGGGTGTGCATAGCCAGACCCTGAGTCGCTTCTTCGATAAAGTGCAGGATCTGGGTCGTGATAGTAAAACCAGCAGTGCAGAAGTTACCATTCAGAATGTTATTAAACTGGTTGATGAGCTGATGGAAGCCCCGGGTGATGTTGAAGCACTGGCCCCGCCGGTGCGTCATCTGATTGCAACCCAGCTGCTGAGCAATCTGGAAGATATTATGCGCATTCTGGCCAAAAGCCTGCCGAAAGGCCCGTTCACCTATATTAGCCCGAGTAATACCGAACTGACCCTGATGATTCAGGAACGCGGTGATAAAAATGTTACCATGGGTCAGAGCAGTGCCCGCATGAAACTGAATTGGGCCGTTGCCGCCGGCGCAGAAGATCCGGGTCCGGCAGTGGCAGGCATTCTGAGTATTCAGAATATGACCACCCTGCTGGCCAATGCAAGTCTGAATCTGCATAGCAAAAAACAGGCCGAACTGGAAGAAATCTATGAAAGTAGTATTCGTGGTGTTCAGCTGCGTCGTCTGAGTGCCGTGAATAGCATCTTCCTGAGTCATAATAATACCAAAGAACTGAATAGCCCGATTCTGTTCGCATTCAGTCATCTGGAAAGCAGTGATGGCGAAGCAGGCCGTGATCCGCCGGCAAAAGATGTGATGCCGGGTCCGCGCCAGGAACTGCTGTGTGCCTTCTGGAAAAGTGATAGCGATCGTGGTGGCCATTGGGCCACCGAAGGTTGCCAGGTTCTGGGTAGTAAAAATGGTAGTACCACCTGCCAGTGTAGCCATCTGAGCAGCTTCGCAATTCTGATGGCCCATTATGATGTTGAAGATTGGAAACTGACCCTGATTACCCGT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~64kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD97 is a member of the EGF-TM7 (seven-span transmembrane) protein family, which is characterized by an extended extracellular region with a variable number of N-terminal EGF-like domains coupled to a TM7 stalk. It is expressed by leukocytes following activation. CD97 binds to its cellular ligand CD55 (decay accelerating factor) and protects several cell types from complement-mediated damage. The CD97-CD55 interaction may play a role in cellular activation, migration and adhesion following inflammation. CD97 expression is increased in thyroid cancer, paralleling dedifferentiation and tumor staging in this disease. Many colorectal cell lines are also CD97+, with CD97 levels correlating with migration and invasion in vitro. CD97 is also expressed in various gastric, pancreatic and esophageal carcinomas. CD97 shares significant homology with EMR2, however the two proteins exhibit different expression patterns, as EMR2 is not expressed in any of the aforementioned cancer cells.

Note:

For research use only, not for use in diagnostic procedure.