Host:

E.coli

Tag:

His-tag

AA Sequence:

CAGGAATTCCTGCTGCGCGTGGAACCGCAGAATCCGGTTCTGAGTGCCGGCGGTAGTCTGTTCGTTAATTGCAGTACCGATTGTCCGAGCAGTGAAAAAATTGCACTGGAAACCAGCCTGAGCAAAGAACTGGTTGCCAGCGGCATGGGTTGGGCAGCATTCAATCTGAGCAATGTGACCGGCAATAGTCGTATTCTGTGTAGCGTGTATTGTAATGGTAGTCAGATTACCGGCAGTAGCAATATTACCGTGTATCGTCTGCCGGAACGCGTGGAACTGGCCCCGCTGCCTCCGTGGCAGCCTGTTGGTCAGAACTTCACCCTGCGTTGCCAGGTGGAAGATGGCAGCCCGCGTACCAGTCTGACCGTGGTGCTGCTGCGCTGGGAAGAAGAACTGAGTCGTCAGCCGGCAGTTGAAGAACCGGCAGAAGTGACCGCCACCGTGCTGGCAAGTCGTGATGATCATGGCGCCCCGTTCAGTTGCCGTACCGAACTGGATATGCAGCCGCAGGGTCTGGGTCTGTTCGTTAACACCAGTGCACCGCGTCAGCTGCGTACCTTCGTTCTGCCGGTGACCCCGCCGCGCCTGGTTGCACCTCGCTTCCTGGAAGTTGAAACCAGTTGGCCGGTGGATTGCACCCTGGATGGCCTGTTCCCGGCCAGTGAAGCCCAGGTGTATCTGGCACTGGGTGATCAGATGCTGAATGCCACCGTTATGAATCATGGCGATACCCTGACCGCCACCGCAACCGCAACCGCGCGTGCAGATCAGGAAGGTGCACGCGAAATTGTGTGTAATGTGACCCTGGGCGGCGAACGTCGCGAAGCACGCGAAAATCTGACCGTGTTCAGCTTCCTGGGTCCGATTGTGAATCTGAGCGAACCGACCGCACATGAAGGCAGCACCGTGACCGTGAGTTGCATGGCAGGCGCACGCGTGCAGGTGACCCTGGATGGTGTGCCGGCCGCCGCACCTGGTCAGCCTGCACAACTGCAGCTGAATGCAACCGAAAGCGATGATGGTCGTAGCTTCTTCTGCAGCGCAACCCTGGAAGTTGATGGCGAATTCCTGCATCGTAATAGTAGCGTTCAGCTGCGTGTGCTGTATGGTCCGAAAATTGATCGTGCAACCTGTCCGCAGCATCTGAAATGGAAAGATAAAACCAGACATGTTCTGCAGTGTCAGGCACGTGGTAATCCGTATCCGGAACTGCGTTGTCTGAAAGAAGGTAGTAGTCGCGAAGTTCCGGTTGGTATTCCGTTCTTCGTTAATGTGACCCATAATGGCACCTATCAGTGTCAGGCCAGCAGTAGCCGCGGCAAATATACCCTGGTGGTGGTGATGGATATTGAAGCAGGCAGCAGTCAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~50kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Cell adhesion molecules (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth. These proteins are thought to play an important role in embryogenesis and development. ICAM-3, also designated CD50 and ICAM-R, is a type I membrane protein that is thought to regulate morphological changes during cell locomotion. ICAM-3 acts as a counter-receptor for the leukocyte Integrin αL/β2, and is known to activate T cells and polymorphonuclear leukocytes. ICAM-3 also binds to Moesin, via the cytoplasmic domain of ICAM-3. The expression of ICAM-3 is induced by RANTES, a chemoattractant known to activate T lymphocytes. ICAM-3 is also a major ligand for the leukocyte Integrin LFA-1 (CD11a/CD18).

Note:

For research use only, not for use in diagnostic procedure.