Host:

E.coli

Tag:

His-tag

AA Sequence:

AAGAAGGTGGTTCTGGGCAAAAAAGGTGATACCGTGGAACTGACCTGTACCGCAAGTCAGAAAAAAAGTATTCAGTTCCATTGGAAAAACAGCAATCAGATTAAAATCCTGGGTAATCAGGGTAGCTTCCTGACCAAAGGTCCGAGCAAACTGAATGATCGCGCCGATAGCCGTCGTAGTCTGTGGGATCAGGGTAACTTCCCGCTGATTATTAAAAATCTGAAAATCGAAGACAGCGATACCTATATCTGTGAAGTGGAAGATCAGAAAGAAGAAGTGCAGCTGCTGGTGTTCGGTCTGACCGCCAATAGTGATACCCATCTGCTGCAGGGTCAGAGCCTGACCCTGACCCTGGAAAGCCCGCCGGGTAGCAGCCCGAGTGTTCAGTGTCGTAGTCCGCGCGGCAAAAATATTCAGGGTGGCAAAACCTTAAGTGTTAGCCAGCTGGAACTGCAGGATAGCGGCACCTGGACCTGTACCGTTCTGCAGAATCAGAAAAAAGTTGAATTCAAAATCGACATCGTGGTTCTGGCATTCCAGAAAGCCAGCAGCATTGTGTATAAAAAAGAAGGCGAACAGGTGGAATTCAGCTTCCCGCTGGCCTTCACCGTGGAAAAACTGACCGGCAGCGGTGAACTGTGGTGGCAGGCCGAACGCGCCAGCAGCAGTAAAAGTTGGATTACCTTCGATCTGAAAAATAAAGAAGTGAGCGTTAAACGTGTTACCCAGGATCCGAAACTGCAGATGGGTAAAAAACTGCCGCTGCATCTGACCCTGCCGCAGGCACTGCCGCAGTATGCCGGCAGTGGTAATCTGACCCTGGCCCTGGAAGCCAAAACCGGCAAACTGCATCAGGAAGTTAATCTGGTTGTGATGCGTGCAACCCAGCTGCAGAAAAATCTGACCTGCGAAGTGTGGGGCCCGACCAGCCCGAAACTGATGCTGAGCCTGAAACTGGAAAATAAAGAAGCCAAAGTGAGTAAACGTGAAAAAGCAGTGTGGGTTCTGAATCCGGAAGCAGGCATGTGGCAGTGTCTGCTGAGCGATAGCGGTCAGGTTCTGCTGGAAAGCAATATTAAAGTTCTGCCGACCTGGAGCACCCCGGTTCAGCCG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~45kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation.

Note:

For research use only, not for use in diagnostic procedure.