Host:

E.coli

Tag:

His-tag

AA Sequence:

GATAGTAGCAAATGGGTGTTCGAACATCCGGAAACCTTATATGCCTGGGAAGGTGCATGCGTGTGGATTCCGTGTACCTATCGCGCACTGGATGGCGATCTGGAAAGCTTCATTCTGTTCCATAATCCGGAATATAATAAGAATACCAGCAAATTCGACGGCACCCGCCTGTATGAAAGCACCAAAGATGGTAAAGTTCCGAGTGAACAGAAACGTGTTCAGTTCCTGGGCGATAAAAATAAAAATTGCACCCTGAGCATTCATCCGGTGCATCTGAATGATAGCGGTCAGCTGGGCCTGCGCATGGAAAGTAAAACCGAAAAATGGATGGAACGTATTCATCTGAATGTTAGCGAACGTCCGTTCCCGCCGCATATTCAGCTGCCGCCGGAAATTCAGGAAAGTCAGGAAGTTACCCTGACCTGTCTGCTGAACTTCAGTTGCTATGGCTATCCGATTCAGCTGCAGTGGCTGCTGGAAGGCGTGCCGATGCGCCAGGCCGCTGTGACAAGCACCAGCCTGACCATTAAAAGTGTGTTCACCCGTAGTGAACTGAAATTCAGTCCGCAGTGGAGCCATCATGGTAAAATTGTGACCTGCCAGCTGCAGGATGCCGATGGCAAATTCCTGAGCAATGATACCGTTCAGCTGAATGTGAAACATACCCCGAAACTGGAAATTAAAGTGACCCCGAGTGATGCAATTGTTCGCGAAGGTGATAGTGTGACCATGACCTGTGAAGTTAGCAGCAGTAATCCGGAATACACCACCGTTAGCTGGCTGAAAGATGGCACCAGTCTGAAAAAACAGAATACCTTCACCCTGAATCTGCGCGAAGTGACCAAAGATCAGAGCGGTAAATATTGCTGTCAGGTTAGCAATGATGTGGGCCCGGGCCGTAGCGAAGAAGTGTTCCTGCAGGTGCAGTATGCACCGGAACCGAGTACCGTGCAGATTCTGCATAGCCCGGCCGTGGAAGGTAGCCAGGTTGAATTCCTGTGCATGAGCCTGGCAAATCCGCTGCCGACCAATTATACCTGGTATCATAATGGCAAAGAAATGCAGGGTCGCACCGAAGAAAAAGTGCATATTCCGAAAATTCTGCCGTGGCATGCAGGTACCTATAGTTGTGTGGCCGAAAATATTCTGGGTACCGGTCAGCGCGGTCCGGGTGCTGAACTGGATGTGCAGTATCCGCCGAAAAAAGTGACCACCGTTATTCAGAATCCGATGCCGATTCGTGAAGGCGATACCGTGACCCTGAGCTGTAATTATAATAGCAGCAATCCGAGCGTGACCCGCTATGAATGGAAACCGCATGGCGCCTGGGAAGAACCGAGTCTGGGTGTGCTGAAAATTCAGAATGTGGGCTGGGATAATACCACCATTGCATGTGCCGCATGCAATAGCTGGTGTAGCTGGGCCAGCCCGGTTGCACTGAATGTTCAGTATGCACCTCGTGATGTGCGTGTGCGTAAAATTAAACCGCTGAGCGAAATTCATAGTGGTAATAGTGTTAGTCTGCAGTGTGACTTCAGCAGTAGCCATCCGAAAGAAGTGCAGTTCTTCTGGGAAAAAAATGGTCGCCTGCTGGGCAAAGAAAGTCAGCTGAACTTCGATAGTATTAGCCCGGAAGATGCCGGTAGCTATAGTTGTTGGGTTAATAATAGCATTGGTCAGACCGCCAGTAAAGCATGGACCCTGGAAGTGCTGTATGCACCGCGTCGTCTGCGTGTGAGTATGAGTCCGGGTGATCAGGTTATGGAAGGTAAAAGTGCAACCCTGACCTGCGAAAGTGATGCAAATCCGCCGGTTAGTCATTATACCTGGTTCGATTGGAATAATCAGAGTCTGCCGTATCATAGTCAGAAACTGCGCCTGGAACCGGTTAAAGTGCAGCATAGCGGTGCATATTGGTGTCAGGGCACCAATAGTGTTGGTAAAGGCCGTAGCCCGCTGAGTACCCTGACCGTGTATTATAGCCCGGAAACCATTGGCCGTCGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~74kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR). After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellular carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation. CD22 has been actively pursued as a therapeutic target for autoimmune diseases. Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for multiple B cell malignancies.

Note:

For research use only, not for use in diagnostic procedure.