Host:

E.coli

Tag:

His-tag

AA Sequence:

TGGGATCAGATGTGCATTGAAAAAGAAGCCAATAAAACCTATAACTGTGAAAATCTGGGCCTGAGTGAAATTCCGGATACCCTGCCGAATACCACCGAATTCCTGGAATTCAGCTTCAACTTCCTGCCGACCATTCATAATCGCACCTTCAGCCGCCTGATGAATCTGACCTTCCTGGATCTGACCCGCTGTCAGATTAATTGGATTCATGAAGATACCTTCCAGAGCCATCATCAGCTGAGCACCCTGGTTCTGACCGGTAATCCGCTGATCTTCATGGCCGAAACCAGTCTGAATGGTCCGAAAAGCCTGAAACATCTGTTCCTGATTCAGACCGGTATTAGCAATCTGGAATTCATTCCGGTGCATAATCTGGAAAATCTGGAAAGCCTGTATCTGGGCAGTAATCATATTAGTAGCATTAAATTCCCGAAGGACTTCCCGGCCCGTAATCTGAAAGTTCTGGACTTCCAGAATAATGCAATTCATTATATCAGCCGCGAAGATATGCGTAGTCTGGAACAGGCAATTAATCTGAGCCTGAACTTCAATGGCAATAATGTGAAAGGCATTGAACTGGGTGCCTTCGATAGTACCATCTTCCAGAGTCTGAACTTCGGTGGTACCCCGAATCTGAGCGTTATCTTCAATGGTCTGCAGAATAGCACCACCCAGAGCCTGTGGCTGGGCACCTTCGAAGATATTGATGATGAAGATATTAGCAGTGCCATGCTGAAAGGTCTGTGCGAAATGAGCGTTGAAAGTCTGAATCTGCAGGAACATCGCTTCAGTGATATTAGCTCAACCACCTTCCAGTGCTTCACCCAGCTGCAGGAACTGGATCTGACAGCAACCCATCTGAAAGGCCTGCCGAGTGGCATGAAAGGCCTGAATCTGCTGAAAAAACTGGTGCTGAGCGTTAATCACTTCGATCAGCTGTGCCAGATTAGCGCAGCAAACTTCCCGAGCCTGACCCATCTGTATATTCGCGGTAATGTGAAAAAACTGCATCTGGGCGTTGGTTGTCTGGAAAAACTGGGTAATCTGCAGACCCTGGATCTGAGCCATAATGATATTGAAGCAAGCGATTGTTGTAGCCTGCAGCTGAAAAATCTGAGCCATCTGCAGACCTTAAATCTGAGTCATAATGAACCGCTGGGTCTGCAGAGTCAGGCATTCAAAGAATGTCCGCAGCTGGAACTGCTGGATCTGGCCTTCACCCGCCTGCATATTAATGCACCGCAGAGCCCGTTCCAGAATCTGCACTTCCTGCAGGTGCTGAATCTGACCTATTGCTTCCTGGATACCAGCAATCAGCATCTGCTGGCCGGCCTGCCGGTTCTGCGTCATCTGAATCTGAAAGGCAATCACTTCCAGGATGGTACCATTACCAAAACCAATCTGCTGCAGACCGTGGGCAGCCTGGAAGTGCTGATTCTGAGCAGCTGCGGCCTGCTGAGTATTGATCAGCAGGCCTTCCATAGCCTGGGCAAAATGAGTCATGTTGATCTGAGCCACAATAGCCTGACCTGTGATAGCATTGATAGCCTGAGCCATCTTAAAGGTATCTATCTGAATCTGGCCGCCAATAGTATTAATATTATTAGCCCGCGTCTGCTGCCGATTCTGAGTCAGCAGAGCACCATTAATCTGTCACATAATCCGCTGGATTGCACCTGCAGTAATATTCACTTCCTGACCTGGTATAAAGAAAATCTGCATAAACTGGAAGGTAGCGAAGAAACCACCTGTGCAAATCCGCCGAGCCTGCGCGGTGTTAAACTGAGCGATGTTAAACTGAGTTGTGGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~66kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD180/RP105 is a type-I transmembrane glycoprotein that is restricted to expression in mature B-cells. Research studies have shown that CD180 engages signaling cascades in mature B-cells that drive cell proliferation. Indeed, CD180 functions with TLR4 as an LPS receptor on B-cells and, upon binding LPS, transmits proliferation and survival signals that require the Vav family of guanine nucleotide exchange factors.

Note:

For research use only, not for use in diagnostic procedure.