Host:

E.coli

Tag:

His-tag

AA Sequence:

AAGAAGGAACATGTGCTGCATTGCCAGTTCAGCGCCTGGTATCCGTTCTTCCGTGGTGTTACCATTAAAAGCGTGATTCTGCCGCTGCCGCAGAATGTTAAAGATTATCTGCTGGATGATGGTACCCTGGTTGTTAGCGGCCGCGATGATCCGCCGACCCATAGTCAGCCGGATAGTGATGATGAAGCAGAAGAAATTCAGTGGAGTGATGATGAGAATACCGCCACCCTGACCGCCCCGGAATTCCCGGAATTCGCAACCAAAGTGCAGGAAGCCATTAATAGCCTGGGTGGTAGTGTGTTCCCGAAACTGAATTGGAGTGCACCGCGTGATGCATATTGGATTGCCATGAATAGTAGCCTGAAATGTAAAACCTTAAGCGATATCTTCCTGCTGTTCAAAAGTAGTGACTTCATTACCCGTGACTTCACCCAGCCGTTCATTCATTGTACCGATGATAGTCCGGATCCGTGTATTGAATATGAACTGGTGCTGCGTAAATGGTGCGAACTGATTCCGGGCGCAGAATTCCGCTGCTTCGTTAAAGAAAATAAACTGATTGGTATCAGCCAGCGTGATTATACCCAGTATTATGATCATATTAGCAAGCAGAAAGAGGAAATTCGTCGCTGCATTCAGGACTTCTTCAAAAAACATATTCAGTATAAGTTCCTGGACGAAGACTTCGTGTTCGATATCTATCGCGATAGTCGCGGTAAAGTGTGGCTGATTGACTTCAATCCGTTCGGTGAAGTTACCGATAGCCTGCTGTTCACCTGGGAAGAACTGATTAGTGAAAATAATCTGAACGGCGACTTCAGCGAAGTTGATGCACAGGAACAGGATAGCCCGGCCTTCCGTTGTACCAATAGTGAAGTTACCGTTCAGCCGAGTCCGTATCTGAGTTATCGTCTGCCGAAAGACTTCGTGGATCTGAGCACCGGCGAAGATGCACATAAACTGATTGACTTCCTGAAACTGAAACGTAATCAGCAGGAAGATGAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~45kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CDC123 required for S phase entry of the cell cycle.

Note:

For research use only, not for use in diagnostic procedure.