Host:

E.coli

Tag:

His-tag

AA Sequence:

GAAACCGTGCATTGCGATCTGCAGCCGGTTGGTCCGGAACGCGGTGAAGTTACCTATACCACCAGCCAGGTTAGTAAAGGCTGTGTTGCACAGGCACCGAATGCCATTCTGGAAGTGCATGTTCTGTTCCTGGAATTCCCGACCGGTCCGAGTCAGCTGGAACTGACCCTGCAGGCCAGCAAACAGAATGGCACCTGGCCGCGCGAAGTTCTGCTGGTTCTGAGTGTTAATAGCAGCGTGTTCCTGCATCTGCAGGCCCTGGGCATTCCGCTGCATCTGGCATATAATAGTAGTCTGGTTACCTTCCAGGAACCGCCGGGTGTTAATACCACCGAACTGCCGAGCTTCCCGAAAACACAAATTCTGGAATGGGCAGCCGAACGTGGCCCGATTACCAGCGCCGCAGAACTGAATGATCCGCAGAGCATTCTGCTGCGCCTGGGCCAGGCCCAGGGCAGCTTAAGCTTCTGCATGCTGGAAGCAAGTCAGGATATGGGTCGCACCCTGGAATGGCGCCCGCGCACACCTGCACTGGTGCGTGGTTGCCATCTGGAAGGTGTTGCCGGCCATAAAGAAGCACATATTCTGCGCGTGCTGCCGGGTCATAGCGCAGGCCCTCGCACCGTTACCGTGAAAGTTGAACTGAGTTGTGCACCGGGTGATCTGGATGCCGTTCTGATTCTGCAGGGTCCGCCGTATGTGAGTTGGCTGATTGATGCAAATCATAATATGCAGATCTGGACCACCGGTGAATATAGCTTCAAAATCTTCCCGGAAAAAAATATTCGTGGCTTCAAACTGCCGGATACCCCGCAGGGTCTGCTGGGTGAAGCCCGTATGCTGAATGCAAGCATTGTTGCAAGCTTCGTTGAACTGCCGCTGGCAAGCATTGTGAGTCTGCATGCAAGCAGCTGCGGTGGTCGTCTGCAGACCAGCCCGGCCCCTATTCAGACCACCCCGCCGAAAGATACCTGCAGTCCGGAACTGCTGATGAGCCTGATTCAGACCAAATGTGCAGATGATGCCATGACCCTGGTTCTGAAAAAAGAACTGGTTGCCCATCTGAAATGTACCATTACCGGCCTGACCTTCTGGGATCCGAGCTGTGAAGCAGAAGATCGTGGCGATAAATTCGTTCTGCGCAGTGCATATAGCAGTTGTGGCATGCAGGTTAGTGCCAGTATGATTAGTAATGAAGCCGTGGTTAATATTCTGAGCAGCAGTAGCCCGCAGCGCAAAAAAGTGCATTGCCTGAATATGGATAGTCTGAGCTTCCAGCTGGGTCTGTATCTGAGTCCGCACTTCCTGCAGGCCAGTAATACCATTGAACCGGGCCAGCAGAGCTTCGTTCAGGTGCGCGTTAGCCCGAGTGTGAGCGAATTCCTGCTGCAGCTGGATAGTTGCCATCTGGATCTGGGCCCGGAAGGTGGTACCGTTGAACTGATTCAGGGCCGCGCCGCCAAAGGTAATTGTGTGAGTCTGCTGAGTCCGAGCCCGGAAGGTGATCCGCGCTTCAGCTTCCTGCTGCACTTCTATACCGTGCCGATTCCGAAAACCGGTACCCTGAGCTGCACCGTGGCCCTGCGTCCGAAAACCGGCAGTCAGGATCAGGAAGTTCATCGTACCGTGTTCATGCGCCTGAATATTATTAGTCCGGATCTGAGTGGTTGTACCAGTAAAGGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~62kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD105/Endoglin is an auxiliary receptor for the TGF-β receptor complex, functioning in related signaling pathways. CD105/Endoglin is a transmembrane protein that exists as a disulfide-linked homodimer. It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells. Upregulated CD105/endoglin expression has been reported during wound healing and tumor vascularization, and in inflammatory tissues and developing embryos. Mutations in CD105/endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure. The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer.

Note:

For research use only, not for use in diagnostic procedure.