Gene/Insert name:

WWP2

Alternative name:

AIP2; WWp2-like

Plasmid description:

Mammalian Expression vector of Homo sapiens WW domain containing E3 ubiquitin protein ligase 2(Identical with the NCBI sequence except for three point mutations G1239A/T1707C/A2091G not causing the amino acid variation) with N terminal 3xFlag tag and the unfused EGFP protein at the C-terminus

Species:

Homo sapiens (human)

Gene ID:

11060

Accessions:

NM_007014.4

Vector backbone:

pIRES2-EGFP

Tag:

3*Flag(N-term);EGFP(C-term)

Vector type:

Mammalian Expression

Backbone size w/o insert:

5300bp

Cloning site 5':

Xho I

Site destroyed during cloning:

NO

Cloning site 3':

EcoR I

Site destroyed during cloning:

NO

Bacterial resistance:

Kanamycin

Growth strain(s):

Top10

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Neomycin