Gene/Insert name:

Usp15(C298S)

Alternative name:

Gcap18; AI327321; 4921514G19Rik; E430033I05Rik

Plasmid description:

Lentiviral expression vector of Mus musculus ubiquitin specific peptidase 15(one point mutation C298S) with the Flag tag and unfused CopGFP protein at the C-terminus(seven point mutation: A1131G/T1191C/T1698C/C1701T/C1707T/T1743G/C2401T not causing the amino acid variation)

Species:

Mus musculus (house mouse)

Gene ID:

14479

Accessions:

NM_027604.4

Vector backbone:

pLenti-CMV-GFP-Puro

Tag:

Flag(C-term);CopGFP(C-term)

Vector type:

Mammalian Expression, Lentiviral

Backbone size w/o insert:

8189bp

Cloning site 5':

Xba I

Site destroyed during cloning:

NO

Cloning site 3':

Not I

Site destroyed during cloning:

NO

Bacterial resistance:

Ampicillin

Growth strain(s):

Stbl3

Growth temperature:

37°C

High or low copy:

High Copy

Selectable markers:

Puromycin